Research on Whole Organ Decellularization Protocols for Kidney Transplantation and Vascular Integrity Evaluation
DOI:
https://doi.org/10.56294/mw2023144Keywords:
Whole-organ decellularization, Kidney transplantation, vascular integrity, Extracellular matrix (ECM) retentionAbstract
The shortage of donor kidneys highlights the need for innovative approaches to transplantation. Whole-organ decellularization creates acellular scaffolds suitable for recellularization with recipient-derived cells, reducing the risk of immune rejection. A key challenge is preserving vascular integrity during decellularization to ensure organ functionality. This aims to compare three decellularization protocols, detergent-based, enzyme-based, and a combined approach, for their effectiveness in maintaining vascular integrity (VI), ECM retention, and perfusion capacity. Additionally, factors influencing VI are analyzed using statistical techniques. Kidneys were processed using the three protocols. VI was measured using parameters such as ECM retention percentage, vascular leakage rates, and perfusion capacity. Pearson correlation analysis determined correlations between parameters such as decellularization time and vascular outcomes. One-way ANOVA contrasted protocol performance, and logistic regression determined predictors of preservation of VI. There were significant differences in ECM retention between protocols. The detergent-based process showed superior ECM retention and reduced vascular leakage compared to other processes. Logistic regression found decellularization time and solution concentration were important predictors of vascular preservation. The detergent-based protocol better maintained VI and ECM retention, thus holding potential for future clinical use in kidney transplantation. Additional optimization might further improve scaffold quality and functional results.
References
1. Zambon JP, Ko IK, Abolbashari M, Huling J, Clouse C, Kim TH, Smith C, Atala A, Yoo JJ. Comparative analysis of two porcine kidney decellularization methods for maintenance of functional vascular architectures. Acta biomaterialia. 2018 Jul 15;75:226-34. https://doi.org/10.1016/j.actbio.2018.06.004
2. Leuning DG, Witjas FM, Maanaoui M, de Graaf AM, Lievers E, Geuens T, Avramut CM, Wiersma LE, van den Berg CW, Sol WM, de Boer H. Vascular bioengineering of scaffolds derived from human discarded transplant kidneys using human pluripotent stem cell-derived endothelium. American Journal of Transplantation. 2019 May 1;19(5):1328-43. https://doi.org/10.1111/ajt.15200
3. Manalastas TM, Dugos N, Ramos G, Mondragon JM. Effect of decellularization parameters on the efficient production of kidney bioscaffolds. Applied Biochemistry and Biotechnology. 2021 May;193:1239-51. https://doi.org/10.1007/s12010-020-03338-2
4. Ciampi O, Bonandrini B, Derosas M, Conti S, Rizzo P, Benedetti V, Figliuzzi M, Remuzzi A, Benigni A, Remuzzi G, Tomasoni S. Engineering the vasculature of decellularized rat kidney scaffolds using human induced pluripotent stem cell-derived endothelial cells. Scientific reports. 2019 May 29;9(1):8001. https://doi.org/10.1038/s41598-019-44393-y
5. Pina S, Ribeiro VP, Marques CF, Maia FR, Silva TH, Reis RL, Oliveira JM. Scaffolding strategies for tissue engineering and regenerative medicine applications. Materials. 2019 Jun 5;12(11):1824. https://doi.org/10.3390/ma12111824
6. Feng H, Xu Y, Luo S, Dang H, Liu K, Sun WQ. Evaluation and preservation of vascular architectures in decellularized whole rat kidneys. Cryobiology. 2020 Aug 1;95:72-https://doi.org/10.1016/j.cryobiol.2020.06.003
7. Hussein KH, Saleh T, Ahmed E, Kwak HH, Park KM, Yang SR, Kang BJ, Choi KY, Kang KS, Woo HM. Biocompatibility and hemocompatibility of efficiently decellularized whole porcine kidney for tissue engineering. Journal of Biomedical Materials Research Part A. 2018 Jul;106(7):2034-47. https://doi.org/10.1002/jbm.a.36407
8. Duisit J, Maistriaux L, Bertheuil N, Lellouch AG. Engineering vascularized composite tissues by perfusion decellularization/recellularization. Current Transplantation Reports. 2021 Jun;8:44-56. https://doi.org/10.1007/s40472-021-00317-2
9. Corridon PR. In vitro investigation of the impact of pulsatile blood flow on the vascular architecture of decellularized porcine kidneys. Scientific Reports. 2021 Aug 20;11(1):16965.https://doi.org/10.1038/s41598-021-95924-5
10. Taylor DA, Kren SM, Rhett K, Robertson MJ, Morrissey J, Rodriguez OE, Virk H, Chacon‐Alberty L, Curty da Costa E, Mesquita FC, Sampaio LC. Characterization of perfusion decellularized whole animal body, isolated organs, and multi‐organ systems for tissue engineering applications. Physiological reports. 2021 Jun;9(12):e14817. https://doi.org/10.14814/phy2.14817.
Published
Issue
Section
License
Copyright (c) 2023 Yogendra Bhati, Biswaranjan Mohanty , Manashree Mane (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The article is distributed under the Creative Commons Attribution 4.0 License. Unless otherwise stated, associated published material is distributed under the same licence.
